Friday 21 June 2013

Response to IL Senate Bill 1666

This is the email I wrote to the Illinois Agriculture and Conservation Subcommittee on Food Labeling yesterday, following the public hearing. I wrote it because the opposition emphasized that the genes inserted into transgenic plants are genes that we commonly consume otherwise, thus implying that there is no harm in eating them in GMO crops. I have sent the email already, but would still really appreciate feedback. I will likely be writing more emails of this nature and hope to get better at it with your help.

Chairman Koehler,

Thank you for sponsoring SB1666 on the labeling of GMO foods. I attended the hearing this morning in Normal out of a strong personal concern for the future of our food supply.

I have a background in technology, having received a Master of Science degree from DePaul in Telecommunications, and a Bachelor of Science degree from UIC in Computer Engineering. I have been a software developer for the past 18 years. A few years ago I experienced some health issues, and launched myself into what I call the "Mother Nature" diet. I emptied my cupboards of processed foods and extracts, preferring to use food in its most natural, whole form. Over time, my body responded to the dietary changes, and with the positive improvements in my health, my interest in nutrition increased. This interest led me to research organic farming methods, and the impact industrial farming has on our ecosystem. Learning about the sources of our food supply led to a deep concern, to the point that I resolved to become involved at a level beyond my own personal interests. I left the world of software behind to return to school, and am now a full time student once again at UIC, working on a Master of Public Health degree in Environmental and Occupational Sciences.

The GMO issue has been especially interesting to me, because of my technical background. In fact, there's a side of me that really hopes that the biotech engineers will one day find success. Although the food industry would like us to believe otherwise, however, success is not yet assured. While the results of independent studies are not conclusive, they do raise sufficient concern over the potential health risks of GMO foods to warrant further study. Unfortunately, long-term impartial studies are not available, leaving consumers, like me, to rely on their own research to make their own judgments for their own health.

I would like to share a few things that I've learned about GMOs that were not addressed in the hearing this morning:

1. The transgene itself is, for me, not the issue of concern; it is rather the process of insertion that poses the greatest risk. While the industry would like us to believe that the insertion of a gene into a new species is a precise operation, it is not. The process most commonly used is called "Transfection by Agrobacterium tumefaciens", and leverages the ability of the bacterium to inject DNA into a cell. The gene is incorporated into the cell's genome at a semi-random location. The resulting location and its ultimate impact on the GM plant are not fully known, even by the technologists supervising the process. Success is declared when the plant expresses the desired trait, but unpredictable side-effects can also result from mutations caused by the insertion, that produce a variety of potentially harmful proteins.

2. The GE technology was initially developed when scientists assumed a one-to-one correspondence between genes and proteins, but we have since learned this is not the case. The DNA "protocol" is very intricate and complex, making the random insertion of a transgene very risky. If the process were equated to software, it would be akin to dropping a small sequence of code randomly into an existing software application. Theoretically, the new portion of code might indeed be useful, but depending on its placement in the application, it could also create havoc, resulting in bugs that might not be realized until much later.

3. Once the insertion is completed, the plant cells are treated with antibiotics to kill off the Agrobacteria before being propagated to crops. Not all are successfully removed, however; some remain on the GM plant to be passed on to future generations; these Agrobacteria can potentially extend the gene insertion to animal cells, or to fungi and yeast, vital parts of the soil ecosystem.

4. The gene is not inserted alone into the plant genome; a stretch of DNA called the "Cauliflower Mosaic Virus" (CaMV 35S) promoter is inserted along with it. This powerful promoter is used to "turn on" the new gene, allowing it to be expressed. This promoter, however, also has the ability and potential to turn on other genes besides the new transgene. It can activate host genes elsewhere in the genome, even on other chromosomes, driving expression of genes in the wrong cells at the wrong time. The insertion of this promoter is of major concern to many scientists.

We are walking a very dangerous line here. Despite the lack of hard evidence against GMOs, many are responding to anecdotal evidence and common sense to steer clear of genetically modified food to protect their own health, as well they should. The lack of transparency by the industry and resistance to disclosure of GMO products adds to the perception of stealth that surrounds the controversy. I do not buy the claims that labeling would increase costs significantly. If GMOs truly add value as they claim, the food industry should be proud to boast about them on their labels.

Thank you for giving me the opportunity to share my perspective. I urge the committee to support SB1666. Labeling of GMO products will help us as consumers to vote for or against this technology, with our food purchases. Labeling will help restore the power of choice to those of us who care about our health and our future.

Respectfully,

Mary Gelder

Westmont, IL 60559

Thursday 28 March 2013

How The Heck Do Transgenes Get Into GMOs?

Two weeks ago I gave a (group) presentation in my biology class on a 1999 paper by Arpad Pusztai on GMOs (Genetically Modified Organisms). Pusztai was leading a group of scientists at the Rowett Institute in Scotland which was developing a test paradigm that would assure the validity and safety of GMO crops, but to their surprise, they discovered serious health effects that could only have been caused by the insertion of the transgene (not the transgene itself, but the process of insertion). Pusztai was appalled to learn that GMO products were already on the market, and when he publicized his findings, the public outrage was so great that major food chains resorted to removing GMO ingredients from their products in Europe, and legislation was introduced throughout Europe for the labeling of GMO products. Of course the biotech industry fought back, discrediting Pusztai, and he was suspended from the Rowett Institute (which does a lot of research funded by the biotech industry). Later, his paper about the research was formally published (under threat) in ‘The Lancet’, which had the paper reviewed by six peers instead of the usual two because of the intensity of the controversy. Five of the reviewers approved publication; one dissented. During this time, although hundreds of articles were published in Europe about the controversy, only two articles were published here in the States. For some reason the media weren’t as interested here.

 
For my presentation I wanted to learn about the process of genetically modifying plants, so I dug in. I had heard about the gene-gun, which blasts genes into the nucleus like a B-B gun, where they usually obliterate the cell altogether. In a few cases (1 in 10,000) the cell survives and the new gene lands in the DNA at some random location. Although they used to use that method more in the past (and still do in some cases), it turns out that in about 90% of the cases now, they use a bacterium (Agrobacterium tumefaciens) to do the dirty work, instead. This is a pathogenic soil bacterium that causes tumors in many species of plants. The tumor-inducing genes in the bacterium are deleted, and the desired gene sequence is cloned in the T-DNA of the bacterium. (That, by the way, is the "precise" part of the process that biotech scientists point to in their defense of GMOs. They don’t bother to share the rest of the story.) The plant seedlings are heated to place them under stress, making them susceptible to the bacteria. The bacteria attach to the cell, and using virulent genes in a biological process known as T4SS (Type IV secretion system), they poke the new gene into the plant cell along with a promoter to turn on transcription. This promoter, known as the Cauliflower Mosaic Virus (CaMV), is one of the most powerful promoters known. Depending on where it lands in the plant’s DNA, it can promote not only the transcription of the new transgene, but other genes downstream or even on other chromosomes. This carries huge unintended risks and is the detail in the GMO process that has scientists very concerned. Although Pusztai’s tests did not identify this as THE element that resulted in the deleterious health effects in the rats he was testing, he suspects that it is likely involved.


As if this weren’t enough, once the transgene is in the plant, the A. tumefaciens bacteria are no longer needed, so the plant cells are treated with antibiotics to kill off the agrobacteria before being propagated to crops. It is about 50% effective; some bacteria survive and are propagated to the crops along with the new cells. Unfortunately, this is yet another routine use of antibiotics, contributing to the serious problem we have of  increasing bacterial resistance to antibiotics.


The bottom line is that there are huge risks with GMOs. Their safety has not been established, and the few independent tests we do have indicate potential for serious health problems. As these problems are not acute (i.e. immediate and severe), but rather chronic (developed over time), it is very difficult to make the association. Researchers are reluctant to take on the biotech giants, so the tests we so desperately need to understand this technology don’t happen.


Here is a 2009 quote from Pusztai, ten years after his controversial paper was published: "One gene expressing one protein is the basis of genetic engineering, but the Human Genome Project discovered [only] 23,000 genes, and there are 200,000 proteins in every cell. With this discovery, genetic engineering should have disappeared into the dustbin."


I feel like I’m only scratching the surface in understanding the depth of this problem, but I thought I would share what I learned in preparing for my presentation. This is nasty business.


My sources:
Viral DNA dangers. (2009, June). Retrieved March 10, 2013, from GM-free Scotland: http://gmfreescotland.blogspot.com/2011/02/viral-dna-dangers.html
Agrobacterium. (2010, December). Retrieved March 10, 2013, from GM-free Scotland: http://gmfreescotland.blogspot.com/2011/04/agrobacterium.html
Agrobacterium tumefaciens. (2013, March 10). Retrieved from Wikipedia, the free encyclopedia: http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens
Gene gun. (2013, March 10). Retrieved from Wikipedia, the free encyclopedia: http://en.wikipedia.org/wiki/Gene_gun
• Boyle, R. (2011, January 24). How To Genetically Modify a Seed, Step By Step. Popular Science. Retrieved March 10, 2013, from http://www.popsci.com/science/article/2011-01/life-cycle-genetically-modified-seed
• Ewen, S. W., & Pusztai, A. (1999, October 16). Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine. The Lancet, 354, 1353-1354. Retrieved March 10, 2013, from http://www.sciencedirect.com.proxy.cc.uic.edu/science/article/pii/S0140673698058607
• Pitzschke, A., & Hirt, H. (2010, February 11). New insights into an old story: Agrobacterium-induced tumour formation in plants by plant transformation. The Embo Journal, 29, 1021-1032. doi:10.1038/emboj.2010.8
• Pusztai, A. F. (1998). SOAEFD flexible Fund Project RO 818. Report of Project Coordinator on data produced at the Rowett Research Institute (RRI), Rowett Research Institute. Retrieved March 14, 2013, from http://www.rowett.ac.uk/gmo/ajp.htm
• Roseboro, K. (2009, June). Arpad Pusztai and the Risks of Genetic Engineering. Organic Consumers Association. Retrieved March 13, 2013, from http://www.organicconsumers.org/articles/article_18101.cfm
• Smith, J. M. (2003). Seeds of Deception. Yes! Books.